Universal Strategy of Efficient Intracellular Macromolecule Directional Delivery Using Photothermal Pump Patch
Tang, Heming1,2, Yang, He1 , Zhu, Wenjun3, Fei, Liyan2, Huang, Jialei1, Liu, Zhuang3, Wang, Lei1,4*（王蕾）, Chen, Hong1*(陈红)
1State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials College of Chemistry Chemical Engineering and Materials Science Soochow University Suzhou 215123, P. R. China
2The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)Hangzhou Institute of Medicine(HIM) Chinese Academy of Sciences Hangzhou, Zhejiang 310022, China
3Institute of Functional Nano&Soft Materials Laboratory(FUNSOM) Soochow University Suzhou, Jiangsu 215123, China
4Jiangsu Biosurf Biotech Company Ltd. Suzhou, Jiangsu 215123,China
Adv. Mater. 2023, 35, 2304365
Abstract：The development of a highly efficient, nondestructive, and in vitro/vivo-applicable universal delivery strategy of therapeutic macromolecules into desired cells and tissues is very challenging. Photothermal methods have advantages in intracellular delivery, particularly in in vivo manipulation. However, the inability of directional transmission of exogenous molecules limits their delivery efficiency. Here, a photothermal pump (PTP) patch with numerous exogenous molecular reservoirs is reported. Under a laser, the cell membrane ruptures, while exogenous molecular reservoirs shrink, resulting in a directional exogenous molecule delivery into cells for a high-efficient intracellular delivery. The PTP patches are considered a universal structure for a highly efficient, nondestructive, and in-vitro/vivo-applicable intracellular macromolecule delivery. Under in vivo transdermal intracellular delivery conditions, the target genes are efficiently and noninvasively delivered into epidermal and dermal cells through the PTP patch and exosomes produced by the epidermal cells, respectively. The PTP patch provides a new strategy for a high-efficiency, nondestructive, and in-vitro/vivo-applicable macromolecule delivery.